Antimicrobial and
Cytotoxic Activities of Zingiber officinalis Extracts
Ayşe NALBANTSOY*,°, Duygu AYYILDIZ TAMİŞ*, I.
Hakkı AKGÜN*, Tansel ÖZTÜRK YALÇIN*, İsmet DELILOĞLU GÜRHAN*,
İsmail KARABOZ**
* Ege University, Faculty of Engineering, Department of
Bioengineering, 35100 Bornova, Izmir, Turkey.
** Ege University, Faculty of Science, Department of
Biology, Basic and Industrial Microbiology Section, 35100
Bornova, Izmir, Turkey.
oCorresponding Author
Summary
In the present study, the edible plant Zingiber officinalis
ethanol and chloroform extracts were prepared and their
cytotoxic effects versus human cervical cancer (HeLa) and
mouse fibroblast (L929) cell-lines were investigated. HeLa
and L929 cell lines in 96 well microplates were cultivated
for 24 h with initial concentrations of 8×04 cells/mL and
7.4×104 cells/mL, respectively. After that the cultures were
treated with different dilutions of the extracts and
incubated for 48 h. The growth inhibition was determined to
the 50% inhibition concentration (IC50). IC50 values were
reported as ±95 % confidence intervals (±95 % CI) by using
Graph Pad Prism (San Diego, CA). The antibacterial activity
of the ginger extracts was tested by the paper disc
diffusion technique. The results of the morphological
observation and MTT test indicated that the cytotoxic
activity of the extracts were dose dependent. IC50 values
versus L929 and HeLa cells were found to be 87.28 μg/mL and
74.32 μg/mL, respectively, for the chloroform extract, while
the ethanol extract showed IC50 values at 101 μg/mL and
33.78 μg/mL, respectively. Moreover, the extracts were
evaluated for their antimicrobial activities against
Klebsiella pneumoniae, Salmonella thyphimurium, Bacillus
cereus, Enterococcus fecalis and Staphylococcus aureus. The
antimicrobial activity results showed that the ginger
extracts inhibited the growth of five out of eight
microorganisms but had no effect on the growth of
Escherichia coli ATCC11230, Pseudomonas aeruginosa ATCC
27853, and Staphylococcus epidermidis ATCC 12228.
Key Words :
Zingiber officinalis, HeLa, L929, anticancer
effect, antimicrobial activity.
