AMPEROMETRIC BIOSENSOR BASED ON MUSHROOM TISSUE TYROSINASE FOR THE DETERMINATION OF PHENOLIC COMPOUNDS

Arzum ERDEM*, Nejat ALTINIGNE*, Emrah KILINÇ**, Levent GÖKGÜNNEÇ**, Tayfun DALBASTI**, Mehmet ÖZSÖZ*°

*Ege University, Faculty of Pharmacy, Department of Food Analysis, Bornova-Izmir, TURKEY.
**Ege University, Faculty of Medicine, Department of Neurosurgery, Bornova-Izmir, TURKEY.
°Corresponding Author

Summary:
An amperometric biosensor based on carbon paste electrode modified with mushroom tyrosinase is defined as a device for quantitative an qualitative detection of phenolic compounds.Potassium ferrocyanide is used as the mediator in the reaction between the mushroom tyrosinase and phenolic compounds.This paper demonstrates the advantage of using mushroom tissues as the biocatalyst in vitro detection of phenolic compounds.Experimental variables such as pH,operating potential and temperature,change in the percentage of the mushroom tissue are discussed.The stability of the mushroom tissue modified electrode is also demonstrated.

Key words:
Amperometry, Tyrosinase, Biosensor, Carbon-paste Electrode, Phenolic Compounds