PROCEDURE FOR DETERMINATION OF AMPHETAMINE AND METHAMPHETAMINE IN URINE BY GC/MS METHOD

Yalçin DUYDU*°

*Ankara University, Faculty of Pharmacy, Department of Toxicology, 06100 Tandogan-Ankara, TURKEY.
°Corresponding Author

Summary :
In the present study a procedure for the dertermination of amphetamine and methamphetamine in urine by Gas Chromatography – Mass Spectrometry (GC/MS) was presented. Amphetamine and methamphetamine were exracted by solid-phase extraction (SPE) and trifluoroacetic anhydride (TFA) was used as the derivatization reagent. The derivatized extracts were analyzed by GC/MS which operated in the selected ion monitoring (SIM) mode. Deuterated analogs of the analytes were used as internal standards. Recovery rates of 93.6% and 94.1% for amphetamine and methamphetamine respectively were obtained.

Key words :
Amphetamine, Methamphetamine, GC/MS Method