HPLC ANALYSIS OF NICOTIANA RUSTICA L. AND CHEWING TOBACCO (MARAS POWDER) ALKALOIDS

Semra KURUCU*°, Murat KARTAL*, Aydin ERENMEMISOGLU**

*Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, 06100 Ankara, Turkey.
**Erciyes University, Faculty of Medicine, Department of Pharmacology, 38039 Kayseri, Turkey
°Corresponding Author

Summary :
A High Pressure Liguid Chromatographic Technique was used to quantitate the nicotine, nornicotine and anabasine leves in Nicotiana rustica L. leaves cultivated in Kahramanmaras and Ankara, and in chewing tobacco (Maras Powder) prepared from Nicotina rustica L. The procedure involved the extraction of the milled tissue followed by “separation and quantitation” of the alkaloids on a reversed phase C18 column with a mobile phase of 40 ? methanol containing 0.2 ? phosphoric acid buffered to pH 7.25 with triethylamine. It was found that the leaves of Nicotiana rustica L. cultivated in Southeast Anatolia and Ankara and chewing tobacco contained nicotine 7.7069, 2.1221, 1.1705 ?, nornicotine 0.1112, 0.0705, 0.0401 ?, and anabasine 0.1538, 0.0341, 0.0579 ? respectively.

Key words:
Nicotiana rustica L., Tobacco Alkaloids, RP-HPLC Analysis.