THE SENSITIVITY TESTS OF BETA-LACTAMASE POSITIVE AND NEGATIVE OF BACILLUS ISOLATES AGAINST CLARITHROMYCIN, AZITHROMYCIN, I AND III CLASS CEPHALOSPORIN AND AMICASIN ANTIBIOTICS

Güven URAZ*°, Mine KAANOGLU*, Ünal KOL*

*Gazi University, Faculty of Science and Arts, Department of Biology, 06500 Besevler, Ankara, TURKEY.
°Corresponding Author

Summary :
A total of, 33 Bacillus strains were isolated from different raw milk samples. In this research, the activity of beta-lactamase enzyme of Bacillus strains was evaluated by lodometric Test and Chromogenic Cephalosporin Test (Nitrocefin). The Bacillus species which were determined as beta-lactamase positive by using lodometric Test and Chromogenic Cephalosporin Test (Nitrocefin) were then controlled by penicillin-G antibiotic. The 10 samples of 33 Bacillus strains determined to be positive for activity of betalactamase enzyme. The two strains of 10 Bacillus strains which showed beta-lactamase positive activity were B.lentus, one strain was B.megaterium, two strains were B.brevis, two strains were B.mycoides and three strains were B.polymxa. The beta-lactamase activity of the remaining 23 Bacillus isolates was found to be negative. One of these was B.brevis, three strains were B.sphaericus, three strains were B.cereus, two strains were B.circulans, two strains were B.coagulans, three strains were B.licheniformis, two strains were B.macerans, one strain was B.mycoides, three strains were B.stearothermophilus and three strains were B.subtilis. Also, the sensitivity tests of beta-lactamase positive and negative Bacillus strains were made against the antibiotics such as azithromycin, clarithromycin, Ist and IIIrd, class cephalosporin and amicasin antibiotics. Beta-lactamase positive and negative Bacillus strains were found to be sensitive to azithromycin and clarithromycin. B.megaterium, B.polymxa-II, B.polymxa-III, B.micoides-I and B.brevis-I strains from 10 beta-lactamase positive Bacillus species were found to he resistant against ceftraxone antibiotic which is a III.class cephalosporine. The others, such as B.lentus-I, B.polymxa-I, B.mycoides-II and B.brevis-II were found to be sensitive. B.lentus, B.polymxa-I, B.mycoides-II and B.brevis-II which were positive of beta-lactamase from Bacillus species were found to be sensitive to cephazoline antibiotic which is a 1st. class cephalosporine. The others were determined to be resistant B.lentus-I. B.lentus-II and B.mycoides-II were found to be sensitive to cephalothine antibiotic. The remaining ones were found to be resistant. Only one strain, B.mycoides-I from the beta-lactamase positive Bacillus strains was determined to be resistant against amicasine antibiotic. The remaining ones were found to be sensitive. All beta-lactamase negative Bacillus strains except for B.licheniformis were found to be sensitive to ceftriaxone antibiotic. B.brevis-III, B.licheniformis, B.stearothermophilus and B.sphaericus from the beta-lactamase negative Bacillus strains were found to be resistant to cephalothine antibiotic, the others were found to be sensitive. Only one strain, B.licheniformis from the beta-lactamase negative Bacillus strains was found to be resistant to cephazoline antibiotic. The others were found to be sensitive. Only one strain, B.cereus from the beta-lactamase negative Bacillus strain, was found to be resistant to amicasine antibiotic.

Key words:
Bacillus, Beta-lactamase enzyme.