SEPERATION OF LEVODOPA, CARBIDOPA AND THEIR RELATED IMPURITIES BY CAPILLARY ELECTROPHORESIS

Selma SARAÇ*°

*Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 06100 Sihhiye, Ankara, TURKEY.
°Corresponding Author

Summary:
In this study a simple and rapid capillary electrophoresis(CE) method was developed for the simultaneous seperation of levodepa, carbidopa and their related impurities. The influence of the pH of the electrolyte on the resolution was also investigated. A baseline seperation was obtained at +20 kV voltage using 40/51 cm (effective/total length) uncoated fused-silica capillary (50?m I.D.) at 20?C in 100 mM phosphate buffer (pH :8.5) as the background electrolyte. Trytophan was used as the internal standard. In these conditions, it is possible to seperate levodopa, carbidopa and their related impurities in short analysis times (less than 8 min).

Key words:
Levodopa and impurities, Carbidopa and impurities, Capillary electrophoresis, Method development.