Hale SAYAN*° , Bilge GÖNÜL*, K. Gonca AKBULUT*, Ali TURKYILMAZ*, Nevin ÇELEBİ**

*Gazi University, Faculty of Medicine, Department of Physiology, Ankara, TURKEY.
**Gazi University, Faculty of Pharmacy, Depatrment of Pharmaceutical Technology, Ankara, TURKEY.
° Correspondence

Summary:
Gastric mucosal injury associated with hepatic dysfunction can be correlated with metabolic perturbation of antioxidan compounds such as reduced glutathione (GSH) and related compounds in the liver and stomach. The aim of this study was to determine the effects of Epidermal Growth Factor (EGF) administration routes on lipid peroxidation (MDA) and glutathione (GSH) metabolism in liver of coldrestraint stress rats. The study was performed on 7 groups:Group I: untreated rats (Control), Group II: Acute cold restraint stress effect on liver tissue (CRS), Group III: Untreated rats after cold restraint stress (CRS-UP), Group IV: Cold restraint stress with intragastric microemulsion treatment (ME), Group V: Cold restraint stress with intragastric EGF treatment in microemulsion (ME-EGF) (for 7 days, 6 ?g/kg) Group VI:Cold restraint stress with intraperitoneal physiologic saline injection (IP-PS), Group VII: Cold restraint stress with intraperitoneal EGF treatment in physiologic saline (IP-EGF)(for 7 days, 6?g/kg).The results indicated the liver MDA levels were significantly increased in all experimental groups.The liver GSH levels of ME-EGF and IP-EGF treated groups were decreased significantly when compared to their control groups.Our results suggest that cold restraint stress caused an increase in hepatic lipid peroxidation levels and EGF treatment decreased hepatic GSH levels.These results might indicate that EGF plays a regulatory role independently to its dosage for or administration route on GSH metabolism in liver.

Keywords:
Cold restraint stress, EGF, Liver, MDA, GSH