Diclehan ÜNSAL*,o , Hale SAYAN** , Bilge GÖNÜL***

*Gazi University, Faculty of Medicine, Radiation Oncology, 06300 Etiler, Ankara, TURKEY.
**Gazi University, Faculty of Medicine, Physiology, 06300 Etiler, Ankara,TURKEY.
***Zonguldak Karaelmas University, Faculty of Medicine, Department of Physiology, Zonguldak, TURKEY.
oCorresponding author

Summary:
As a result of preoperative radiotheraphy, impaired or delayed wound healing may be a frequent clinical problem. Wound healing is characterized by synthesis of collagen. Histamine and ascorbic acid have important effects on collagen synthesis.In this controlled, randomized study; it was aimed to evaluate the effects of GM-CSF applied topically on the preoperatively irradiated woud healing in the context of ascorbic acid and histamine content. Half the rats were irradiated as 8 Gy total body irradiation and 2 days after radiotherapy, they were randomized into 4 groups: group 1- control, group 2- GM-CSF applied group, group 3- irradiated control group, group 4- irradiated and GM-CSF applied group.11 days after radiotherapy tissue samples were taken. Histamine content of the wound was found to increased 320% by irradiation (p=0.002) and decreased 81% by drug administration in the irradiated wound (p=0.002). The ascorbic acid content was decreased 51% by irradiation and GM-CSF made no change on its level. The results show that GM-CSF diminished the increase histamine content of irradiated wound. Further investigations are required to determine the mechanism involved.