Summary :
Pantoprazole is a gastric hydrogen-potassium adenosine triphosphatase (H+ /K+ ATPase) inhibitor. In this study spectrophotometric methods have been developed for the determination of pantoprazole in its tablet dosage forms. Pantoprazole in methanol - water (1:9, v/v) solution was determined at the wavelength ranges of 200-350 nm by the two spectrophotometric methods. Analysis was performed at 295 nm and 303 nm for UV and first derivative UV spectrophotometric methods, respectively. Linearity ranges were found as 2.50 - 80.00 µg mL-1 for UV spectrophotometric method and 0.5-70 µg mL-1 for first derivative UV spectrophotometric method. Limits of quantitation were determined as 2.31 µg mL-1 and 0.5 µg mL-1 and limits of detection as 0.69 µg mL-1 and 0.15 µg mL-1 for UV and first derivative UV spectrophotometric methods, respectively. Developed methods were validated and showed good precision and accuracy. The proposed methods were successfully applied to the assay of pantoprazole in pure and tablet dosage form. No interference was found from tablet excipients at the selected wavelengths and assay conditions. The data were compared with those obtained from the spectrophotometric method given in the literature and no difference was found statistically.