QUANTITATIVE DETERMINATION OF DISULFIRAM-CONTAINING PHARMACEUTICALS BY IR SPECTROSCOPY AND HİGH PRESSURE LIQUID CHOROMATOGRAPHY METHODS

Berna ÖKÇELİK*, Okan ATAY*,o

*Gazi University, Faculity of Pharmacy, Department of Pharmacetucal Chemistry, 06330 Hipodrom, Ankara, TURKEY.
oCorresponding author e-mail:okatay@gazi.edu .tr

Summary:
In this study, infrared (IR) spectroscopic and high pressure liquid chromatographic (HPLC) methods are described for quantitative determination of disulfiram (DSF) in solid dosa¬ge form. IR spectroscopic method (KBr disc technique) was used and dehydrocholic acid (DHCA) as inteenal standard. The spe¬cific absorption bands at 914 and 1705 cm-1 were chosen for DSF und DHCA respectively. Beer’s Law was obeyed in the con¬centration range of 0.4-1.2% w/w in KBr. At 914 cm-1, regres¬sion equation was found to be y=0.2267x + 0.1287 (r=0.9976)
In HPLC method, DSF and Me.frusid (MFD) (internal standard) were separated by isocratic system in that mobile phase consis¬ted of methanol-phosphate buffer (pH=3±0.1) (80:20 v/v). Fluw-rate was 0.7 ml. min-l and Luna 5?C18 (250 x 4.6 mm) was used as stationary phase. Linear concentration was obta¬ined as 1-4mcg mL-1. The regression equation was found to be y= 5.1504x - 0.272 (r= 0.9989)

Keywords:
Disulfiram, IR, HPLC, Quantitative determina¬tion.