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FABAD
J. Pharm. Sci.
ISSN 1300-4182
Copyright Ó 2005
FABAD. All rights reserved
FABAD
J. Pharm. Sci., 29(1), 15-19, 2004. PDF
(76 KB)
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Research
Articles
ABSTRACT
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MALONDIALDEHYDE QUANTIFICATION IN
BLOOD PLASMA OF TOBACCO SMOKERS AND NON-SMOKERS
Suna ATASAYAR * , Hilmi ORHAN * , Hilal ÖZGÜNEÞ *,o
*Hacettepe University, Faculty of Pharmacy, Department of
Pharmaceutical Toxicology, 06100 Sýhhiye, Ankara, TURKEY.
oCorresponding Author
Summary
We adapted the thiobarbituric acid assay (TBAA) in our
laboratory and validated it according to the study conditions
in order to quantify plasma malondialdehyde (MDA) concentration.
MDA-thiobarbituric acid (TBA) derivative was synthesized
as a reference compound and characterized by UV visible
and fluorescence spectra. Calibration curves of the spectrophotometric
assay were linear (r2 = 0.979-0.999) over a concentration
range of 2.5-10 µM. The limit of detection was 1.1 µM.
Within-day coefficient of variation (CV) for pooled human
plasma samples was 8.2%, and between-day variation was
17.3%. The accuracy of the assay for the standard concentrations
of 2.5, 5.0, 7.5 and 10.0 mM was calculated as 94.1%,
90.0%, 91.3%, and 94.7%, respectively. The assay was further
applied to the fresh plasma samples of male human smokers
(n=10) and their age- and sex-matched counterparts (n=10).
The plasma levels were found as 6.7±0.2 µM (mean± SEM),
and 4.9±0.1 µM (mean±SEM), respectively. The difference
was statistically significant (p < 0.05).
Key Words :
Malondialdehyde, validation, spectrophotometry, blood
plasma, human.
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