DETERMINATION
OF DIFLUNISAL IN TABLETS USING DERIVATIVE UV SPECTROPHOTOMETRIC
METHODS
Filiz SAYIN*o, Sedef KIR*
*Hacettepe University, Faculty of Pharmacy,
Department of Analytical Chemistry, 06100 Sýhhiye,
Ankara - TURKEY.
oCorresponding Author
Summary
In this study, derivative UV spectrophotometric methods
were developed for the analysis of diflunisal, which is
used as a nonsteroidal anti-inflammatory drug. Analysis
wavelengths of diflunisal for the first and second order
derivative UV spectrophotometric methods were obtained as
235.6 nm and 228.8 nm, respectively. The linearity ranges
were found as 1.0-25.0 µg mL -1 for the first order
and 1.0-20.0 µg mL -1 for the second order derivative
UV spectrophotometric method. The detection limit was 0.1
µg mL -1 for both developed methods. Derivative UV
spectrophotometric methods were also applied to the synthetic
samples and tablets containing diflunisal. The results of
tablet analysis, which were obtained by derivative UV spectrophotometric
methods, were compared with spectrofluorimetric method in
the literature. No statistically significant difference
was found between diflunisal concentrations obtained from
the three methods. The mean percentage recoveries and mean
relative standard deviations were found to be 100.00% and
0.27% for the first order and 99.94% and 0.26% for the second
order derivative UV spectrophotometry. The precision and
accuracy of the methods were found to be lower than 2% by
statistical evaluation.
Key Words :
Diflunisal, derivative UV spectrophotometry,
tablet analysis, spectrofluorimetry.
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