Evaluation of the Viability of L-929 Cells in the Presence of Alendronate and Absorption Enhancers
 Fulya KARAMUSTAFA*, Nevin ÇELEBİ*^,°, Zelihagül DEĞİM*, Şükran YILMAZ**
* Gazi University, Faculty of Pharmacy, Department of Pharmaceutical Technology, 06330, Etiler, Ankara, Turkey
** Food and Mouth Diseases Institute, 06520, Eskisehir Way, Ankara, Turkey

^oCorresponding Author

Summary

Alendronate and two absorption enhancers (dimethyl-β-cyclodextrin and sodium taurocholate) were selected as model
substances. The cytotoxicity of alendronate and the absorption enhancers was evaluated on mouse fibroblast cells (L-929) by using MTT assay. Higher concentrations of alendronate and the absorption enhancers demonstrated greater toxicity for L-929 cells. Serum presence in culture medium decreased the toxicity potential. Longer period of exposure of the cells to the substances as a consequence of extended experiment period also decreased the cell viability.

Key Words :
Alendronate, sodium taurocholate, dimethyl-β-cyclodextrin, L-929, MTT test.