Comparison of Staphylococcal Beta-lactamase Detection Methods

Ekrem KILIÇ*^,°, Meltem YALINAY ÇIRAK**
* Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Sihhiye, Ankara, TURKEY
** Gazi University, Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Besevler, Ankara, TURKEY

^oCorresponding Author

Summary

Antimicrobial and antimalarial activities of 22 secondary metabolites isolated from the methanolic extracts of Verbascum cilicicum Boiss., Verbascum lasianthum Boiss. ex Bentham and Verbascum pterocalycinum var. mutense Hub.-Mor. (Scrophulariaceae) were investigated. Growth inhibition, using microdilution method, was determined against Candida albicans ATCC 90028, Cryptococcus neoformans ATCC 90113, Staphylococcus aureus ATCC 29213, methicillin-resistant S. aureus ATCC 43300, Pseudomonas aeruginosa ATCC 27853, Aspergillus fumigatus ATCC 90906 and Mycobacterium intracellulare ATCC 23068. Amphotericin B, ciprofloxacin and rifampin were used as controls. It was found that ilwensisaponin A and C showed antimicrobial activity against Aspergillus fumigatus ATCC 90906, but no activity was seen against Gram (+) and Gram (-) bacteria and the yeasts used in this study. None of the other compounds had antimicrobial activities. The antimalarial activities of the compounds were also tested to Plasmodium falciparum clone [Sierra Leone D6 and W2 (chloroquine-sensitive)]. The antimalarial agents chloroquine and artemisinin were used as controls. None of the compounds showed antimalarial activities.

Key Words :
Ilwensisaponin A, ilwensisaponin C, Verbascum cilicicum, Verbascum lasianthum, Verbascum pterocalycinum var. mutense, Scrophulariaceae, antimicrobial activity, antimalarial activity