Electrochemical Evaluation of Pioglitazone HCl and its Determination in Pharmaceutical Dosage Forms
 Uğur AĞBABA*, Sibel A. ÖZKAN*^,o
 * Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06100, Ankara, TURKEY

^oCorresponding Author

Summary

The voltammetric behavior of Pioglitazone was studied using cyclic, linear sweep, differential pulse (DPV) and square wave voltammetric (SWV) techniques. The oxidation of Pioglitazone was irreversible and exhibited a diffusion-controlled process dependent on pH. The dependence of peak current and potentials on pH, concentration, scan rate, and nature of the buffer was investigated. Different parameters were tested to optimize the conditions for the determination of Pioglitazone. According to the linear relation between the peak current and the concentration, DPV and SWV methods for its quantitative determination in pharmaceutical dosage forms were developed. The optimum conditions for analytical applications were obtained. Using optimized DPV and SWV techniques, the current was linear within a concentration range of 6x10^-6 M and 2x10^-4 M with a detection limit of 1.66x10^-6 M and 1.12x10^-6M, respectively, in pH 3.16 phosphate buffer. The repeatability, reproducibility, precision and accuracy of the methods were also investigated. These methods were utilized for the determination of Pioglitazone in tablets. No electroactive interferences from the excipients were found in the pharmaceutical dosage forms. The results obtained from tablet dosage forms were compared with each other and found in a good agreement between DPV and SWV techniques.

Key Words :
Pioglitazone, voltammetry, oxidation, pharmaceutical analysis, validation.