development and valıdatıon of uv spectroscopy method for determınaton of amlopıdıne besylate ın spıked plasma wıtout derıvatızatıon
 Murat ÖZTÜRK*, Yücel KADIOĞLU*,^o
* Atatürk University, Faculty of Pharmacy, Department of Analytical Chemistry, 25240, Erzurum, Turkey

^oCorresponding Author

Summary

A rapid and sensitive UV spectroscopic method is described for the determination of amlodipine besylate (ADB), a calcium channel blocker, in plasma. The sample preparation was a simple extraction with ethanol-acetonitrile (1/3) solvent mixture in buffer medium. The type of solvent, range of wavelength and range of concentration were chosen in order to optimize the conditions. The method was successfully applied to the analysis of ADB in spiked plasma samples. The standard solutions and plasma samples were prepared in ethanol-acetonitrile (30:70, v/v) solvent mixture. The calibration curves were linear (r > 0.994) in concentration range of 2-17 µg ml^-1 of ADB. The limit of quantification (LOQ) and limit of detection (LOD) values of the method for plasma samples were 2 µg ml^-1 and 1.5 µg ml^-1, respectively. The precision calculated as the relative standard deviation (RSD) was less than 2%, and accuracy (relative error) was better than 6% (n=6). The developed method in this study can be directly and easily applied for the determination of ADB in spiked plasma without derivatization.

Key Words :
Amlodipine besylate, UV spectroscopic method, plasma, validation.