Nanoparticle and Liposome Formulation of Doxycycline and Investigation of Transport Properties Through Caco-2 Cell Lines
 Çiğdem YÜCEL*°, Zelihagül DEĞIM**, Şükran YILMAZ***
 * Erciyes University Faculty of Pharmacy, Dept. of Pharm. Technology, Kayseri, Turkey
 ** Gazi University Faculty of Pharmacy, Dept. of Pharm. Technology, Ankara, Turkey
 *** Food and Mouth Diseases Institute, Ankara, Turkey

 ° Corresponding Author E-mail: cyucel@erciyes.edu.tr

Summary

Controlled release systems are nowadays successfully used for treating diseases. Nanoparticles and microparticles are
used to improve the quality of life, to decrease drug dosages, to prolong dosage intervals and are used for protection from harmful side effects. Glioblastoma multiforme is the most common, disruptive and fast growing malign tumor in the
central nervous system. Doxycycline is a type of tetracycline antibiotic that plays a key role in the growth and spread of
the tumor as it inhibits the MMP-2 enzyme and produces an antitumor effect. In this study, doxycycline liposome and nanoparticle formulations were prepared. In order to determine the various concentrations of the different substances to be used in different formulations, MTT tests were performed, penetration specifications of the cell cultures of doxycycline Caco-2 were investigated, by comparing the release kinetics of the formulations. Doxycycline encapsulation efficiencies were calculated as 67.4% for the liposomes, whereas this figure for the prepared nanoparticles was calculated as 53.4%. As the result of the MMP-2 test, inhibition percentage of the doxycycline liposomes was found to be 96%, which is thought to help to stop the dissemination of the glioblastoma cells.

 

Key Words
Doxycycline, Nanoparticle, Liposome, Glioblastoma multiforme.