Simultaneous Estimation of Cefixime and Dicloxacillin in Bulk and Tablet Formulation by RP-HPLC Method
 Somnath D. BHINGE*°, Sharangouda M. MALIPATIL**, Lalit V SONAWANE***, Hariprasanna
R.CHITTAPURKAR*
* RMES’s College of Pharmacy, Gulbarga, India.
** HKES’s College of Pharmacy, Gulbarga, India.
*** SCES’s Indira College of Pharmacy, Tathwade, Pune-33, India
° Asst. Prof., Dept. of Pharmaceutical Chemistry, RMES’s College of Pharmacy, Old Jewargi Road, Balaji Nagar,
Gulbarga –585102, (Karnataka), India
Corresponding Author E-mail: somu1245@yahoo.co.in
 
 

Summary

Present study reports development and validation of simple,economic, selective, precise, and accurate RP high-performance liquid chromatography (RP-HPLC) method for the analysis of cefixime and dicloxacillin in bulk drug and pharmaceutical formulations. The developed method has shown good resolution for cefixime, dicloxacillin and formulation excipients present in tablets. A mixture of 5 mM Phosphate Buffer (pH 5.4 adjusted with ortho-phosphoric acid): acetonitrile: methanol (42: 55: 3 v/v/v) was used to elute the drug component. HPLC analysis of cefixime and dicloxacillin was carried out at a wavelength of 225 nm with flow rate of 1.0 mL min–1. The method was quantitatively evaluated in terms of linearity, precision, accuracy (recovery), selectivity and robustness as per standard guidelines. The method is simple, convenient and suitable for the analysis of cefixime and dicloxacillin in bulk and pharmaceutical formulations.

Key Words :
Reverse-phase HPLC, Cefixime, Dicloxacillin, ICH guidelines.