Comparison of the Alchemilla L. Samples from
Turkish Herbal Market with the European
Pharmacopoeia 8.0
Gülin RENDA*°, Ferhat TEVEK**, Büūra KORKMAZ*, Nurettin YAYLI*
* Karadeniz Technical University, Faculty of Pharmacy, Department of Pharmacognosy, 61080-Trabzon, TURKEY
** Karadeniz Technical University, Faculty of Pharmacy, 61080-Trabzon, TURKEY
° Corresponding Author;
Tel: +90 462 377 8830,
E-mail: grenda@ktu.edu.tr, gulingurhan@yahoo.com
Summary
The aims of this study are; to investigate the morphological properties
of Alchemilla samples which are purchased from Turkish herbal
market and to examine the suitability to the pharmacopoeia,
according to the monograph of A. vulgaris which is registered
as official species in European Pharmacopoeia. 13 samples were
collected from different herbal market in Turkey, afterwards
morphological analyses were carried out. Foreign matter and loss
on drying analyses, total ash quantities and TLC analyses were
performed according to the monograph in European Pharmacopoeia
8.0. Zones which were obtained with the chlorogenic acid and cafeic
acid solutions compared with the zones of sample extracts in the TLC
chromatograms. It was determined that the samples examined belong
to different species from A. vulgaris. Also volatile organic compounds
of the samples were determined by a solid phase micro extraction
(SPME) method coupled with gas chromatography-flame ionization
detector (GC-FID) and gas chromatography-mass spectrometry
(GC-MS). Aldehydes were found as the major group of compounds
in all of the samples in the range of 49.7% - 93.4% ratio. The
major volatile constituents of the samples were found to be hexanal
(in the range of 15.2%-29.0%) excluding sample 9 of which the
major compound was furfural (18.8%). It was determined that none
of the investigated samples meet the requirements of the European
Pharmacopoeia 8.0 monograph.
Key Words :
Alchemilla, GC-MS, monograph, pharmacopoeia,
Rosaceae, herbal market
