DETERMINATION OF MORPHINE IN HUMAN HAIR BY GC/MS METHOD

Yalçin DUYDU*°

*Ankara Universty, Faculty of Pharmacy, Department of Toxicology, 06100, Tandogan-Ankara, TURKEY.
°Corresponding Author

Summary:
In this study morphine was determined in hair obtained from two persons suspected of heroine abuse by Gas Chromatography-Mass Spectrometry (GC/MS). Morphine were extracted by solid-phase extraction (SPE) and derivatized with N,O,-bis (Trimethylsilyl) trifluoroacetamide (BSTFA) +1% Trimethylchlorosilane (TMCS). The derivatized extracts were injected into the GC/MS which operated in the selected ion monitoring (SIM) mode. Linearity was observed in our working range with “R2” value of 0.9983. The recovery rate of the extraction method was 91.8% for morphine. 45.19 and 12.5 ng morphine/100 mg hair were determined in sample A and sample B respectively using this method.
Key words:
Morphine, Hair, GC/MS.