History
 

FABAD  J. Pharm. Sci.
ISSN 1300-4182
Copyright Ó 2005 FABAD. All rights reserved 

FABAD J. Pharm. Sci., 28(1), 39-49, 2003.PDF (318 KB)

Scientific Reviews

ABSTRACT

PERFUSED LIVER PREPARATION AND ITS APPLICATIONS

Selma ŞAHİN *,o

* Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Technology, 06100 Ankara, TURKEY.
oCorresponding Author

Summary:
Unlike most organs of the body, the liver has a dual blood supply.Under physiological conditions, one third of its blood is supplied by the hepatic artery with the remainder coming from the portal vein.The liver has prompted numerous investigators not only to define the anatomical and functional relationship between these blood vessels but also to study various hepatic functions.The hepatic functions have been studied using various experimental models including isolated organelles, isolated and cultured hepatocytes,liver slices and perfused liver. In comparison to the in vitro models,as the structural and functional integrity of the organ is retained in the perfused liver system,this experimental model is widely used for physiological, pharmacological and pharmacokinetic investigations. In this review the perfusion system, perfusion medium and its characteristics,perfusate flow rate and pressure, the live donors, anaesthetic agents, surgical procedures and viability assessment of he perfused liver will be described, and finally its applications will be briefly presented.