PERFUSED
LIVER PREPARATION AND ITS APPLICATIONS
Selma
ŞAHİN *,o
* Hacettepe
University, Faculty of Pharmacy, Department of Pharmaceutical
Technology, 06100 Ankara, TURKEY.
oCorresponding Author
Summary:
Unlike most organs of the body, the liver has a dual blood
supply.Under physiological conditions, one third of its
blood is supplied by the hepatic artery with the remainder
coming from the portal vein.The liver has prompted numerous
investigators not only to define the anatomical and functional
relationship between these blood vessels but also to study
various hepatic functions.The hepatic functions have been
studied using various experimental models including isolated
organelles, isolated and cultured hepatocytes,liver slices
and perfused liver. In comparison to the in vitro models,as
the structural and functional integrity of the organ is
retained in the perfused liver system,this experimental
model is widely used for physiological, pharmacological
and pharmacokinetic investigations. In this review the
perfusion system, perfusion medium and its characteristics,perfusate
flow rate and pressure, the live donors, anaesthetic agents,
surgical procedures and viability assessment of he perfused
liver will be described, and finally its applications
will be briefly presented.