Evaluation of
Antioxidant Activity of Various Herbal Folk Evaluation
Medicine
Ece Miser-SALIHOGLU*°, Galip AKAYDIN**, Emel Calıskan-CAN*,
Sevgi Yardım-AKAYDIN*
* Gazi University, Faculty of Pharmacy,
Department of Biochemistry, Turkey
** Hacettepe University, Faculty of Education, Department of
Science and Mathematics for Secondary Education, Turkey
oCorresponding Author:
ecemiser@gazi.edu.tr
Summary
This study was prepared to determine natural antioxidant
capacity of some plants, which grow in Düzce and its
surroundings, and are used as folk medicine. For this
purpose, we investigated Smilax excelsa L., Mespilus
germanica L., Equisetum telmateia Ehrh. , Urtica dioica L.,
and Laurocerasus officinalis Roemer plants and upper parts
of the soil and leaves of these plants were extracted
separately. In order to determine the antioxidant capacity
of these plants, different in vitro methods such as
N,N-dimethyl-p-phenylenediamine (DMPD·+) radical scavenging
activity, cupric ion (Cu2+) reduction capacity, lipid
peroxidation inhibiting activity were applied. Also phenolic
contents of these plants were measured by Folin Ciocalteu’s
method. Vitamin C, gallic acid, and trolox were used as the
reference antioxidant compounds. As a result of our study,
we have found that while Smilax excelsa, Laurocerasus
officinalis and Urtica dioica have relatively high content
of phenolic compounds, Equisetum telmateia and Mespilus
germanica have relatively low content of phenolic compounds.
In general Smilax excelsa and Laurocerasus officinalis had
higher antioxidant activity.
Key Words :
Antioxidant activity; phenolic content; folk
medicine.