Method Development and Validation of Metformin and Repaglinide in Rabbit Plasma by RP-HPLC
Ramanjireddy TATIPARTHI, Dhachinamoorthi DURAISWAMY, Chandrasekhar KOTHAPALLI BANNOTH
* Department of Pharmaceutics, Jimma University, P.O. Box 378, Jimma, Ethiopia.
** Department of Pharmaceutics, QIS College of Pharmacy, Andhra Pradesh, India.
*** Department of Chemistry, Jawaharlal Nehru Technological University Anantapur, Andhra Pradesh, India.

° Corresponding Author E-mail: rmnjrddy@gmail.com

Summary

A sensitive, specific and rapid high-performance liquid chromatography-ultraviolet spectroscopic (HPLC-UV) method was developed and flourishingly validated to estimate the metformin and repaglinide in rabbit plasma. The solvent extraction method was used for obtaining metformin and repaglinide from serum, using ethyl acetate and 0.1N hydrochloric acid. The mobile phase includes acetonitrile:phosphate buffer pH 4.0 at 60:40% v/v with 1% triethylamine at the flow rate of 0.8mL/min and at fixed wavelength of 254 nm. On ten minutes of run time, metformin was retained at 5.1 and repaglinide at 7.4 min. Extraction efficiency was 98% for metformin and 95% for repaglinide. The intra-day and inter-day precision was, in terms of relative standard deviation (RSD), less than 1.76% for both compounds, in the same column and 4.55% in different columns. The developed method was validated, and this proposed method can be used further for pharmacokinetics studies.

Key Words :
RP-HPLC method, plasma, metformin, repaglinide.