Optimization Study of Coumarın 7-hydroxylase Enzyme Activity in Mouse Brain and Liver
Elçin Deniz ÖZDAMAR*, Benay Can EKE*^,°
* Ankara University Faculty of Pharmacy Toxicology Department, Ankara, Turkey
° Corresponding Author E-mail: eke@pharmacy.ankara.edu.tr

Summary

In this study, the optimum conditions (pH, amount of protein, substrate concentration, incubation time) of a cytochrome P450 dependent enzyme, coumarin 7-hydroxylase (COH), activity were determined in microsomal fractions of mouse liver and brain. It’s indicated that different reaction conditions are required for this enzyme activity in mouse liver and brain microsomes. The results obtained, reveal the requirement of optimization studies on COH activity for different tissues of mouse.

Key Words :
Mouse, liver, brain, CYP450, coumarin 7-hydroxylase.