DNA Damage and Lipid Peroxidation in Several Types of Cancer
Emel CALISKAN-CAN*°, Ece MISER-SALIHOGLU*, Can ATALAY**, Ulku YALCINTAS-ARSLAN***, Bolkan SIMSEK*, Sevgi YARDIM-AKAYDIN**

 

* Gazi University, Faculty of Pharmacy, Department of Biochemistry, Ankara, Turkey
** Ankara Oncology Training and Research Hospital, Department of General Surgery, Ankara, Turkey
*** Ankara Oncology Training and Research Hospital, Department of Medical Oncology, Ankara, Turkey
° Corresponding Author E-mail: emelcan@gazi.edu.tr

Summary

Reactive oxygen and nitrogen species (ROS-RNS) might cause formation of lipid peroxidation (LP), DNA damage, and destruction of membrane structure by attacking membrane lipids. DNA damage and lipid peroxidation leads to carcinogenesis related mutagenesis. In our study, we aimed to investigate 8-hydroxy-2’-deoxyguanosine (8OHdG) levels as a product of DNA damage, and malondialdehyde (MDA) levels as a product of lipid peroxidation, which cancer related oxidative stress markers. 8OHdG levels in urine were measured by a competitive ELISA kit method and normalized with creatinine, and MDA levels in serum were measured by HPLC method. Twenty one cancer patients and 21 control subjects were included in the study. Patients were classified as colon cancer (n=12), gastric cancer (n=6), and breast cancer (n=3). 8OHdG/creatinine levels were 26.78±13.56 nM/mM in total patients, and 21.60±7.12 nM/mM in healthy controls (p>0.05), and MDA levels were 3.32±0.85 μM in total patients, and 2.35±1.02 μM in healthy controls (p=0.002). Significantly increased levels of MDA were observed in colon and gastric cancer groups (p=0.048 and p=0.007, respectively). We did not find any correlation between 8OHdG and MDA. In conclusion, increased levels of MDA and, 8OHdG support the studies suggesting possible participation of oxidative stress in cancer. In addition, the results of our study support the studies which suggested that polyunsaturated fatty acids are more susceptible to reactive oxygen species than other components such as DNA or proteins in cancer.

Key Words :
DNA damage, 8-hydroxy-2’-deoxyguanosine, lipid peroxidation, malondialdehyde, cancer.