Development of method for the determination of lead in teeth
samples by flow injection hydride generation atomic
absorption spectrometry in the presence of K3Fe(CN)6, HNO3
and NBH4
Aysel BERKKAN*,º, Duygu Fersun BÝNGÜL*, Mehmet
Tevfik ORBEY*
* Gazi University, Faculty of Pharmacy, Division of
Analytical Chemistry, 06330, Hipodrom Ankara, Turkey
° Corresponding Author E-mail: ayselberkkan@gmail.com
Summary
Flow-injection hydride generation (FI-HG), coupled with
atomic absorpsion spectrometry (AAS) was used for the
determination of lead (Pb) at teeth samples. Plumbane (PbH4)
from acid solution was generated by the reaction of
potassium ferricyanide (K3Fe(CN)6) and sodium
tetrahydroborate (NaBH4). Forty teeth samples were analyzed
and Pb levels were found between 0.66 and 15.60 μg g–1.
Characteristic sensitivity and limit of detection value were
found to be
0.6 mg L–1 and 0.4 mg L–1 respectively. The precision of the
method in terms of relative standard deviation, R.S.D%, for
11 replicate measurements of blank signal was found as 3.8%.
External calibration graph was linear between 10- 60 mg L–1
Pb at 283.3 nm wavelength. The accuracy of the method was
tested using standard reference material (SRM), NIST-SRM
1468 Bone Meal. The results were in good agreement at 95%
confidence level statistically.
Key Words :
Lead hydride, plumbane, tooth, atomic
absorption spectrometry.
