The Effects of Iodine Deficiency on Pulmonary Xenobiotic Metabolizing Enzymes in Rats

Pınar ERKEKOĞLU*, Belma KOÇER-GÜMÜŞEL*°,
Aydan ÇAĞLAYAN*, Filiz HINCAL*,°

*Hacettepe University, Faculty of Pharmacy, Department of Toxicology, Ankara 06100 TURKEY

°Corresponding authors:
Filiz HINCAL, Belma KOÇER GİRAY
Hacettepe University, Faculty of Pharmacy,
Department of Toxicology, Ankara 06100 TURKEY
Tel: +90 312 309 29 58; Fax: +90 312 311 47 77
E-mail: fhincal@hacettepe.edu.tr; bgiray@hacettepe.edu.tr

Summary

The objective of this study was to investigate the effects of iodine deficiency on pulmonary xenobiotic metabolizing enzyme systems in rats. Three-week old male Wistar rats were fed for seven weeks. Iodine deficiency was produced by sodium perchlorate containing drinking water. The total microsomal cytochrome P450 (CYP450) and cytochrome b5 (CYP b5) levels; activities of microsomal NADPH-cytochrome P450 reductase (P450R), microsomal aniline hydroxylase (CYP2E1), microsomal ethoxyresorufin O-deethylase (EROD), microsomal pentoxyresorufin O-depenthylase (PROD) and cytosolic glutathione S-transferase (GST) were determined. Significant decreases in the level of total CYP450; in the activities of P450R, CYP2E1 and GST; increases in the level of CYP b5 and the activities of EROD and PROD were observed. Overall results of this study have indicated the occurrence of significant alterations in pulmonary xenobiotic metabolism in iodine deficient pubertal rats.

Key Words :
Iodine deficiency, xenobiotic metabolizing enzymes, cytochrome P450, cytochrome b5, NADPH-cytochrome P450 reductase, aniline hydroxylase.