Short-Term Resveratrol Pretreatment Suppresses Noradrenaline-Induced Contractions in Human Saphenous Vein and Internal Mammary Artery Rings

Özlem RAKICI*, Sait AŞLAMACI**, Fatma AKAR*°

*Department of Pharmacology, Faculty of Pharmacy, Gazi University;
**Department of Cardiovascular Surgery, Faculty of Medicine, Baskent University, Ankara, Turkey.
°Corresponding Author:
Phone + 90-312 2023127;
Fax: +90-312 2235018
E-mail address: fakar@gazi.edu.tr

Summary

The aim of the present study was to investigate whether short-term resveratrol pretreatment has a suppressive effect on the contraction to noradrenaline and whether this changes between human saphenous vein (SV) and internal mammary artery (IMA). The effects of endothelium removal and pretreatments with nitric oxide synthase inhibitor (L-NOARG) and cyclooxygenase inhibitor (indomethacin) were studied to explain the mechanism of action of resveratrol. SV and IMA samples were obtained from 38 patients undergoing coronary artery bypass operation. The contractile response-curves for noradrenaline (10-8-10-4 M) were obtained in SV and IMA rings. Pretreatment with resveratrol (10 and 30 mM) for 20 min significantly suppressed the contractions to noradrenaline in both vessel rings with endothelium. L-NOARG pretreatment completely abolished resveratrolinduced suppression on the contractions in SV, but not in IMA. Moreover, resveratrol has also inhibitory effect on the contractions in endothelium denuded rings from IMA. Resveratrol of 30 mM suppressed noradrenaline-induced maximal contractions by 40 % in both SV and IMA rings. This effect of resveratrol was mainly endothelium-dependent and mediated by nitric oxide in SV as different from that of IMA. The pretreatment with resveratrol could be a new method for preparation of grafts and prevention of vasospasm.

Key Words :
Short-term resveratrol pretreatment, noradrenaline, contraction, human vessels