HPLC ANALYSIS OF PSORALEN FROM PSORALEA ACAULIS STEV. AND PSORALEA BITUMINOSA L.

M. Levent ALTUN*°, Nevin TANKER**

*Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, 06100 Tandogan, Ankara, TURKEY. **Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Botany, 06100 Tandogan, Ankara, TURKEY.
°Corresponding Author

Summary :
A High Pressure Liquid Chromatographic technique was used to determine Psoralen, on a reversed phase C18 column, using methanol/water (21:10) in the leafy branches, flowers, fruits and roots of Psoralea acaulis Stev. and Psoralea bituminosa L.
In the present study, the amounts of Psoralen in the leafy branches, flowers, fruits, roots of P. acaulis were 0.2107 %, 0.3118 %, 0.1641 %, 0.1016 % and of P. bituminosa 0.0673 %, 0.1566 %, 0.0708 %, 0.0771 % respectively.

Key words:
Psoralen, Psoralea acaulis Stev., Psoralea bituminosa L., RP-HPLC Analysis.