Devrim DEMİR*, Filiz ÖNER*,°, Tanıl KOCAGÖZ**

* Hacettepe University,Faculty of Pharmacy,Department of Pharmaceutical
Biotechnology,06100,Ankara,TURKEY.
**Hacettepe University,Faculty of Medicine,Departmant of Clinical Microbiology and
Microbiology,06100,Ankara,TURKEY
°Corresponding author

Summary:
In this study effects of CaCl2 concentration and incubation temperature on the E. Coli transformation by plasmid DNA complexes were investigated. Three pDNA formulations (pDNA,pDNA +Ziwittergent® 3-08, pDNA:Cationic Lipid (1:1 w/w) + Ziwittergent®3-08) were examined. The formulation containing plasmid DNA : cationic lipid complex (1:1 w/w) and Ziwittergent® 3-08 showed better transformation efficiency. 50 mM CaCl2 concentration and 37-40ºC incubation temperatures showed positive effects on the number of transformed E. coli cells.